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BACTEC™ // I. PRINCIPLE. The detection of microorganisms in a patient’s blood has diagnostic and prognostic importance. Bactec Plus blood culture bottles were preincubated at 35°C or at room temperature before entry into the Bactec instrument to determine the influence of. The overall recovery of organisms and time to detection with the BACTEC and BACTEC systems were compared in a multicenter.

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Bactec Blood Culture System, BD Bactec

It is interesting that the five isolates not detected by the Bactec aerobic media were all NAC species, including one C.

The six different blood culture bottles were each inoculated with 1, yeasts per bottle and then incubated in the corresponding automated system. The views expressed are those of the authors and do not reflect the official policy or position of the Department of the Army, Department of Defense, or the U. Most of the Candida spp.

A suspension of each specimen was made in 5 ml of normal saline and adjusted to a 0. After all, as long as one bottle of a standard aerobic and anaerobic blood culture set detects Candida growth, the clinician will be able to treat the patient appropriately.

It is used for detecting the amount of bacterial contamination in platelet concentrates. Author information Article notes Copyright and License information Disclaimer. Growth detection results for each Candida spp.


Conversely, all Candida spp. The growth requirements and characteristics may be different than C. Growth was detected in all BacT and Bactec mycology bottles, all BacT aerobic bottles, and by terminal subculture of all bottles.

Is it actually important for both the aerobic and anaerobic media to detect Candida growth? Make a Call Today! Aerobic, anaerobic, and mycology media were evaluated in each automated blood culture system. The BacT aerobic medium detected C.

Bactec 9240 Blood Culture System, BD Bactec 9240

Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard. Terminal subculture was performed on all 65 of the bottles that had no growth detected by the automated blood culture systems; all of these cultures demonstrated heavy growth of pure yeast colonies, representing 65 false-negatives.

The ability of automated blood culture systems to detect growth of NAC species has not been thoroughly evaluated. Harrison2 92400 Duane R. Controlled clinical comparison of three commercial blood culture systems. Under these circumstances, when only aerobic and anaerobic media are used, the BacT performed better than the BACTEC in overall detection, time to detection, number of false-negatives, and missed episodes of simulated candidemia.

Please review our privacy policy. However, these studies address only a small bactex of candidemia episodes, primarily secondary to Candida albicans.

Please select a Capability in order to start your service request. Use of simulated blood cultures to compare a specific fungal medium with a standard microorganism medium for yeast detection.


Apart from offering enhanced safety and simplified baxtec, it delivers excellent performance, media quality, reliability, and ease of use.

BD BACTEC™ Blood Culture Media – BD

The mean time to growth detection of C. All aerobic and anaerobic bottles were inoculated with 10 ml of blood, while mycology bacte were inoculated with 5 ml of blood, per the manufacturer’s recommendations. Therefore, time to detection of Candida growth is an important consideration. However, few institutions routinely utilize a mycology bottle as a part of all blood culture sets, secondary to the requirement for extra blood and bacteec space.

Ward for assistance with statistical analysis of the bctec. The mean time to growth detection in the BacT system was Additionally, the routine use of mycology media adds a substantial expense; each mycology bottle is approximately three times more expensive than an aerobic or anaerobic blood culture bottle. Fifty isolates of Candida spp. If a standard 5-day incubation period were employed, the Bactec would have missed an additional episode of C.

This unique medium contains a lysing agent to lyse both red and white blood cells, releasing any intracellular organisms.