Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.
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Collagen-based biomaterials in the form of sponges bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, collagem control and DPPA sponges respectively were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage.
Glycosaminoglycans accumulated in the matrix, up to 1. Vacanti Journal of cellular biochemistry. Showing of 71 extracted citations. Have you forgotten your login?
Gelatinase A activation is regulated by the organization of the polymerized actin cytoskeleton. Metals Organ Ceramics Chitosan polylactic acid-polyglycolic acid copolymer. In order to validate and quantitate the extent of reaction, the degree of cross-linking was determined by the measure of the hetbage primary amino group content of the samples. Ham A Benghuzzi Biomedical sciences instrumentation Treatment of native bovine pericardium with 0.
Daniel Herbage 1 AuthorId: BiomaterialsElsevier,22, pp. Development and characterisation of a three-dimensional in vitro hepatic fish model to investigate xenobiotic metabolism Matthew G Baron Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering. Both supports were always present during the study and a partial decrease in size and weight was detected only with control sponges, both seeded and unseeded.
Anne-Marie Freyria 1 AuthorId: In this report, we propose a simpler, faster way collaggen prepare acyl azide groups and to cross-link collagen-based biomaterials, using diphenylphosphorylazide DPPA as a reagent. Recently, we described an efficient cross-linking process via the formation of acyl azide groups on methylated carboxyl groups of collagen using hydrazine and nitrous acid referred to here as the hydrazine method.
With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices. Herabge deposition Glycosaminoglycan deposition Articular cartilage repair Collagen-based biomaterial Collagen synthesis. Methods for the treatment of collagenous tissues for bioprostheses.
References Publications referenced by this paper. AliDavid J Mooney Current topics in microbiology and immunology These results were corroborated by resistance to bacterial collagenase digestion: In the 10 7 cells-seeded sponges, mineral deposition, observed with unseeded sponges, was significantly decreased 2- to 3-fold.
Specific cartilage collagens herbzge II and XI were deposited in the matrix throughout the culture and traces of type I collagen were noticed only in the culture medium after weeks and 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively. Cross-linking of dermal sheep collagen using a water-soluble carbodiimide.
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Showing of 84 references. BonassarCharles A. Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture. Howard The Journal of biological chemistry Gelatine-Based Biomaterials and Their Biocompatibility: