Kleihauer-Betke (KB) Test. The KB test is performed to quantitate the number of fetal cells present in the maternal circulation. Once the size of the feto-maternal. Hemoglobin F quantitation by flow cytometry has been found to be simple, reliable, and more precise than the Kleihauer-Betke test,87 In the CAP. The Kleihauer-Betke test is a somewhat crude laboratory method used to screen maternal blood samples for the presence of fetal red blood cells.

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RBC count Hematocrit Hemoglobin. Kleihauer test, showing fetal red blood cells in rose-pink color, while adult red blood cells are only seen as “ghosts”. Reticulocyte index Haptoglobin Mentzer index.

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However, if the mother and fetus are ABO incompatible, it is more crucial to quickly perform the KB stain following a stillbirth, as the fetal red blood cells will be eliminated from the maternal bloodstream very quickly, causing the KB stain to underestimate the degree of FMH, if any. None of the trauma scoring systems predicted PTL. However, there are times when the additional dose is necessary due to massive red blood cell FMH and subsequent maternal immune response.

Improvement of the Kleihauer-Betke test by automated detection of fetal erythrocytes in maternal blood.

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The Rosette test is a qualitative screening test performed on a maternal blood sample to determine if FMH has occurred between a Rh-positive fetus and a Rh-negative mother and serves as a useful screening test. The volume of fetal bleed is then calculated kleihaueer determine how much additional RhIG is required. A standard blood smear is prepared from the mother’s kleihsuer and exposed to an acid bath. Review Detection of fetomaternal hemorrhage. Trauma is the number one cause of pregnancy-associated maternal deaths in the United States Ref: Am J Public Health.

This dose will suppress the immune response to 2. Home Products Most Popular Contact. J Obstet Gynaecol Can. Their records were abstracted for demographics, injury type, three separate trauma scores, documented uterine contractions, PTL contractions with progressive cervical changeand serious perinatal complications.

Cytometry B Clin Cytom.

Kleihauer–Betke test – Wikipedia

Log In Sign up for our mailing list. Reasoning that the magnitude of TPH would reflect uterine injury, we evaluated Kleihauer-Betke testing as an independent predictor of preterm labor PTL after maternal trauma. This is when the Kleihauer-Betke KB test is essential. The red arrows indicate maternal cells, which appear as ghost cells.

Clinical assessment does not. See Potential Diagnosis section for preliminary Rosette testing.

Prevention of Rh alloimmunization. TPH, defined as KB-positive for greater than 0. The KB test is performed to quantitate the number of fetal cells present in the kleihaer circulation. In general this is not a problem.


Kleihauer-betke testing is important in all cases of maternal trauma.

Cortey A, Brossard Y. Sign up for LabCE’s mailing list. No items in your cart. During this incubation period, any Rh-positive fetal cells in the maternal sample are sensitized to the anti-Rho D immune globulin and bound. Guidelines for the Management of a Pregnant Trauma Patient.

Kleihauer-Betke (KB) Test – , Laboratory Continuing Education

For a complete discussion of the justification for testing for FMH and methods of estimating the percentage of fetal blood volume lost, see WiSSPers, volume 1, number 1.

Affilations 1 Medical University of South Carolina. Blood drawing can be done pre- or postpartum at the convenience of the care provider and the mother; only if cesarean section is anticipated klehauer it important to draw the sample prior to delivery. Because this is an independent risk factor, many authors recommended routine use in the setting of trauma, regardless of Rh status and regardless of the mechanism or force of the trauma.

Standard Kleihauer-Betke testing in any experienced laboratory is sufficient. From Wikipedia, the free encyclopedia.

Kleihauer Betke Test – StatPearls.