Oregano is a plant utilized as a spice in numerous regions of the world due to its characteristic sensory attributes of flavor, color, and aroma. The use of oregano. This study evaluated growth patterns of Mexican oregano (Lippia berlandieri) under salt stress and tested the hypothesis that this species is salt tolerant. J Food Prot. Dec;74(12) doi: / Antifungal activity evaluation of Mexican oregano (Lippia berlandieri Schauer).
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Thus, adequate pH values can change the membrane permeability and disintegrate the outer membrane of Gram-negative bacteria releasing lipopolysaccharides. Therefore, future investigations should be conducted for the application of CMC and starch as edible coatings with MOEO in food to confirm their antibacterial effect and mechanical and sensory properties.
Inhibition curves were fitted to the Fermi model. Microbial modeling has allowed estimating key intrinsic factors as pH and MOEO concentration with the synergistic effect against two important food-borne pathogens. Carvacrol 5-isopropylmethylphenol and thymol 2-isopropylmethylphenol are the major components of the essential oils of some species belonging to the Lamiaceae family including oregano, and these are phenolic compounds; isomers of monoterpenes exhibit significant antimicrobial activity in vitro [ 67 ].
Table of Contents Alerts. Listeria monocytogenes and Staphylococcus aureus Fermi model biological parameters values at different concentrations of Mexican oregano Lippia berlandieri Schauer essential oil and pH values added to CMC and starch edible films. This is an open access article distributed under the Creative Commons Attribution Licensewhich permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Correspondence should be addressed to Raul Avila-Sosa ; xm. In this case, two main components of MOEO are thymol and carvacrol whose pH dissociation values are acidic 4.
Moreover, these authors pointed out that EO effectiveness is influenced by the sensitivity of the microorganism. The antimicrobial activity of essential oils and plant extracts is well known for a long time, and many research results have been published against food-borne pathogens [ 1 ] and about their low toxicity to mammals, fewer environmental effects, and low cost, which make them more attractive than synthetic antimicrobials [ 2 ].
The data used to support the findings of this study are available from the corresponding author upon request. Materials and Methods berlajdieri. The aim of this work was to evaluate the effect of pH and Mexican oregano essential oil concentration added to CMC and S edible films on Listeria monocytogenes and Staphylococcus aureus.
Significant differences were found in and values.
Inhibition curves of Listeria monocytogenes at different concentrations of Mexican oregano Lippia berlandieri Schauer essential oil and pH values added to CMC a 0. In addition, its chemical, physical, and functional characteristics make them suitable to add essential oils EOs as antimicrobials [ 10 — 12 liplia. The obtained spectra were compared with the respective mass spectra of pure compounds and with the mass verlandieri of the same compounds available from the US National Institute of Standard Technology NIST library.
Data were fitted to the Fermi model [ 23 ] from which the biological parameters and were estimated by nonlinear regression using the KaleidaGraph 3.
Films were made according to the Bertuzzi et al. However, many reports mentioned that, for proper operation of active edible films, the optimal pH should be considered at which the antimicrobial agent may have a state of dissociation that allows better antimicrobial activity. Among them, carboxymethyl cellulose CMC and starch S are the most widespread and economic biomaterials. Microbial Strains of L. Antimicrobial assay was performed. To receive news and publication updates for Journal lippiq Food Quality, enter your email pippia in the box below.
Moreover, there are evidences that EOs incorporated to edible films can inhibit a large variety of food-borne pathogens [ 13lippka — 19 ]. Several works lippiz the literature focus on the evaluation of the antibacterial effect of EOs and propose them as an interesting option to ensure the safety and quality of minimally processed foods and extend their shelf life [ 1314 ].
Inhibition curves of Staphylococcus aureus at different concentrations of Mexican oregano Lippia berlandieri Schauer essential oil and pH values added to CMC a 0.
The main advantage of using EOs in a polymer matrix coating is a slow diffusion of the antimicrobial agent, leaving high concentrations of the active compound in contact with the food surface where contamination has occurred or is likely to occur, for a long period [ 20 ]. Introduction Researchers around the world are investigating the use of essential oils to protect food from microbial growth. MOEO was incorporated at 0. Researchers around the world are investigating the use of essential oils to protect food from microbial growth.
In this report, the kind of polymer chain and pH affects the release of antimicrobial MOEO compounds due to many factors such as electrostatic interactions, osmosis, structural changes, and environmental conditions [ 7 berlanideri, 32 ].
San Claudio, Ciudad Universitaria, Col. Moreover, the Fermi model can explain and predict microbial reduction in all tested pathogens and is suitable to describe inactivation in similar matrices and under different operating conditions in the future [ 26 ]. Based on their traditional use, these extracts obtained from plants are first recommended, due to their broad-spectrum antimicrobial action and low side effects treatments [ 3 ].
Subscribe to Table of Contents Alerts. Essential oils also have the additional effect of maintaining the quality of the food during storage, including sensorial, nutritional, and functional properties [ 45 ]. Therefore, CMC releases more active compounds. Edible Film Preparation Films were made according to the Bertuzzi et al. Currently, research has been focused on the use of edible films due to their safety regarding consumption and friendly relation with the environment, and coating materials being used currently include polysaccharides cellulose derivatives, starch, chitin, and gumsproteins soy, milk, gelatin, corn zein, and glutenand lipids oils, waxes, and resins [ 9 ].
Then, sorbitol Aldrich Chemical Co. Natural antimicrobial compounds effectiveness depends on the type, genus, species, and strain of the target microorganism and some food intrinsic and extrinsic factors such as pH, temperature, water content, atmospheric composition, and initial microbial load, with pH being one of the most important factors due to antimicrobial dissociation [ 3 ]. All tests were run in triplicate.